31 January 2018 2 3K Report

Hi all,

I had to make some endo-tox free plasmid but there is still some ethanol in the final eluted volume.I added 80 ul TE water for a total eluted volume of 200 ul.I stored them at -20 but it is still not frozen after 20 hours. Does ethanol have any negative affect on Transfection in HELA? I am planning to centrifuge them again at 15000 g for 30 mins at 4 degree. But, I am not sure whether I would get a pellet or not due to the dilution factor after the addition of water.

What should I do now? Should I proceed?

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