EDTA metal complexes in water?

More Stefano Salvatori's questions See All

Black dots "bubbling" at cell surface?

Hi, I observed a strange behaviour in my cell lines I had never seen before. Those are stable lines from Ovarian cancer (line's name: HEY) and from Prostate cancer (line's name: PC-3). Both lines...

08 April 2024 1,418 0 View

LCA calculation on raw materials textile use ?

Dear friends, I am Stefano Cipriani and dong an ecolabel that will be useful for the market, especially to increase use of recycled materials. I have a question that would love to share here in...

27 March 2024 5,298 3 View

The twin-effect in water cannot be different than the one in vacuum, what are the consequences on Lorentz Transformations?

Consider two clocks set just under the surface of water, they are synchronized. One of them departs at velocity v and then rejoins at -v. The overall time dilation must be the same as the one in...

14 March 2024 2,203 86 View

Does BFA keep ROS inside of cells?

I recently discovered that BFA is used in flow cytometry, enabling the observation of intracellular cytokines that would be otherwise released. Is the same principle applicable to ROS? I would...

04 March 2024 7,094 1 View

Can ip E.coli injection cause death in 4 hours?

Does an ip E.coli injection (10^8 ) cause death in 4 hours in C57BL/6? Do I need to hydrate the mouse? Is this a good concentration to mimic a septic situation?

04 March 2024 4,552 1 View

How do the power-split and compound architectures of hybrid electric vehicles (HEVs) work?

I'm searching for a comprehensive textbook or paper on power-split and compound architectures in hybrid electric vehicles (HEVs). I've come across some very interesting papers on HEVs. However,...

11 February 2024 8,516 0 View

Micrometer calibration reference method?

Good day, may you suggest a reference method for micrometers calibration? Thanks in advance Stefano

06 February 2024 8,027 3 View

Could anyone help to identify this TC contamination?

Hi ResearchGate community, can anyone help us out with this contamination? We work with iPSC-NPC. This contamination happens ONLY when we put the neuronal progenitors in coverslips + media for...

14 December 2023 6,435 0 View

What is the subsample size for a confusion matrix?

Dear all, do you know what is the correct subsample size (number of pixel) to carry out a confusion matrix? I am digitizing the true classes on an image to check the accuracy of the classifier....

11 August 2023 6,528 2 View

Has anyone had problems with the PyroMark Q48 cartridges?

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23 July 2023 8,854 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

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10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

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10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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09 August 2024 9,621 0 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Stefano Salvatori

Thank you Hayder!

Vinesh Kumar

we determine the total hardness of water using EDTA. If you need this protocol the i will send it to you.

Abhinav Maheswaran Pragatheeswaran

I want to find the exact amount of Chelating agent (eg. EDTA) required to trap all the metal ions in my sample. I do not want any extra (unused) chelating agent in the sample. Is there any technique to find the minimum amount of chelating agent required ? Thanks :)

Supriya Singh

Metal EDTA complex are characterised by blue color of Patton and Reeder indicator in basic medium of pH 12. Therefore make the solution basic using sodium hydroxide solution, add a pinch of P&R indicator and add slowly EDTA solution. The color change from Red to Blue with indicate the complete chelation.