Does anyone have an experience with antibodies that detect both phosphorelated and unphosphorylated forms of proteins?

Dera all, I'm searching for antibodies that detect phosphorelated form of protein and I found some antibodies that recognize both phosphorelated and unphosphorylated forms of proteins. I just...

04 May 2020 8,639 3 View

Quantum mesh in Multi-junction solar cell at the SILVACO?

I designed a multi-junction solar cell in the Silveco Atla, which has two tunnel regions. But when I use a quantum mesh for these regions, my simulation is having trouble. Can anyone guide me?

04 May 2020 2,121 3 View

Is there any GAMS source code for solving multi-objective optimization problems (Aggregate Production Planning)?

I also have question about Energy consumption, is there any way to find each product energy consumption? Is the cost of Energy consumption increases by increasing production (number of products)?

21 September 2019 4,256 1 View

Fe-Mn-Al-Ni Shape memory alloys chemical composition?

Dear researchers, Do you think that the shape memory effect of this alloy on this chemical composition is achievable?

17 February 2019 6,953 2 View

Up to what percentage of similarity is acceptable for a graduate thesis in academic engineering programs?

The zero percentage of similarity is very ideal and most journals accept 10% to 30%, but what about the graduate engineering thesis?

28 January 2019 322 1 View

How to update boundary condition in each time step in comsol?

I have a model for electrophoresis of a particle,I need to update boundary condition in each time step. Actually I calculate boundary condition (velocity of wall) from dependent variables that...

19 January 2018 3,509 4 View

How to calculate hydrodynamic and electrostatic force acting on a particle in Comsol?

I want to model forces acting on single chared collide in nanochannel with charged walls. electrical potential diffrence set along the nanochannel and cause electroosmosis flow in channel. main...

16 December 2017 9,938 1 View

If the sample size is 322, is it right to use Smart PLS for analyzing data?

The sample size of my research is 322.Since the distribution of my data wasn't normal, I used Smart PLS to analyze data.But I heard that the sample size must be at most 200 to use Smart PLS. Is it...

28 August 2016 9,927 5 View

Mixed ANOVA or Two-way ANOVA?

If I have to count the bacterial load of 3 different stations in a lake along time (4 seasons), how should I analyse the results? Should I use Mixed ANOVA or 2-way ANOVA? Any other suggestion and why?

27 April 2016 3,662 9 View

What is the best way to reprobe another antibody on a western blot?

Currently I'm using "Restore plus western blot Stripping Buffer" for reprobing a new antibody on my blots. I am not that happy with the results because the residues of first use are still there!

02 March 2014 2,985 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View