EBV-LCL cell line not growing well?

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Is it possible to do simulation for laser ablation in water using COMSOL or Lumerical?

Solving heat transfer equation during laser ablation in air is done with comsol and there are some tutorial videos, is it possible to do do simulation for laser heating during ablation in liquid....

15 April 2024 9,515 1 View

How to find the value for MAGMOM?

Hi. I am working on a project with VASP with MoO3 lattice and recently I found out that I have to consider spin polarization in my calculations. So, now I do not know what value I should consider...

24 September 2023 4,559 2 View

Treatment of Stacking Pad in lateral flow test????

Hi everyone! I want to use a cellulose pad as Stacking Pad in my Lateral Flow test. how can i treat this pad??? Thanks for any help!

31 July 2023 2,791 2 View

How could we avoid aggregation of gold nanoparticles during antigen conjugation??

Hi Despite considering the suitable pH of the target antigen for conjugation with the help of NAOH, after adding the target antigen to PH-adjusted gold solution, and after adding 1% BSA, and...

25 July 2023 2,600 2 View

How could we avoid aggregation of gold nanoparticles during antigen conjugation??

Hi Despite considering the suitable pH of the target antigen for conjugation with the help of NAOH, after adding the target antigen to PH-adjusted gold solution, and after adding 1% BSA, and...

25 July 2023 3,779 3 View

How to calculate atom positions in Primitive orthorhombic lattice?

Hi everyone, I need to add an acetone to a catalyst surface with the primitive orthorhombic structure and I need a help to how predict the atom positions manually (without the help of VESTA or...

29 June 2023 1,730 1 View

How to find current topics related to interior architecture ?

Current topic & new topic & hot topic

23 June 2023 6,675 1 View

How can we, as an interior architect, change people's behavior and mood?

Whats your idea about that ...

07 May 2023 2,203 2 View

How can I remove a research from my ResearchGate account?

I wanted to delete a research from my ResearchGate account, but instead of deleting it from ResearchGate, I deleted it from my profile. Now how can I remove from researchgate considering that it...

03 April 2023 1,578 4 View

In GARCH models, both the error term and the variance term are squared, while the errors can be negative, so how can it be combined with ARIMA models?

It is said that in order to combine garch models with arima models, their results are added together, while garch results are positive in every step. Is there anything wrong with this combination?...

19 February 2023 6,643 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View