To generate a fragment intended for ligation with sticky ends, I will restriction digest a designed gBlock. This will result in the fragment that I'm after (~600 bp), and 10 bp fragments which were on both ends of the large fragment and were cut during the digestion.
I was wondering if there is a more efficient way to separate and recover the 600 bp fragment than gel electrophoresis and gel extraction.
Any suggestions?
Dear Aiko
a simple pcr purification kit ad for example promega ah wizard pcr clean up (https://ita.promega.com/resources/protocols/technical-bulletins/101/wizard-sv-gel-and-pcr-cleanup-system-protocol/) where the coloums are designed to separate pcr fragments from primers ( fragments
Dear Aiko,, I agree with Manuele Martinelli regarding simple and efficient way to recover 600 bp, but be carefull, you can lost a part of your product. If it is crucial for you electroelution after agarose gel it is the best way.
I agree with Manuele: kits for PCR clean-up will be fine. I have been using Macherey Nagel's Gel and PCR clean-up kit with good success
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