In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

How to rotate crystal structure to match with match another structure of same compound/polymorph?

Say, I have two crystal structures of a particular organic molecule, the crystal structures are basically identical, apart from a rotation and redefinition of the lattice vectors/angles, a simple...

07 January 2021 3,406 5 View

What acid can be used to dissolve limestone to extract microfossils?

I am trying to extract possible Loriciferans from mudstone, attempts with acetic acid (15%) and chloridric acid (37%) have failed to dissolve the rock, would a higher concentration (50%) of HCl...

17 December 2020 5,897 7 View

Is there any cheap spacer on nucleotide (other than photocleavable) that can be cut off chemically?

07 December 2020 1,730 2 View

What does a higher LoD indicate in context of COVID19?

I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...

28 November 2020 1,238 3 View

Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

How did these marble-like limestone stones formed?

There is a small section of a beach south of Tiros, Greece where most of the gravel is made up of these marble-like stones. While I was there suddenly several waves came ashore, but the sea was...

26 October 2020 5,797 4 View

Do you know any diagnosis that is based on fluorescence intensity of marked cells ?

I am a physicist, so I know the techn not the biological applications. The question is straightforward. I am looking for a diagnosis that rely on fluorescent based cell imaging where the intensity...

25 October 2020 1,653 3 View

What assays / application would benefit the most from absolute quantitative fluorescence measurement ?

Many assays, including Cell-based assays, QPCR, some ELISA test, rely on the presence/absence of fluorescence. Diagnosis applications about cancer or antiobiotic resistance uses fluorescence ratio...

21 October 2020 1,159 3 View

I need info on particular Convolvulaceae pollen, can you help?

Dear colleagues in palynology, paleobotany, and botany I desperately need information/papers on the pollen morphology of the following Convolvulaceae genera: Astripomoea, Calycobolus,...

14 October 2020 8,216 10 View