Is there any available dataset that has COVID-19 symptoms values ?

I need a dataset which has real values of COVID-19 patients symptoms such as cough and temperature? I have found some datasets online but it contains only 0 or 1 for the symptoms values? Anyone...

29 January 2021 2,232 3 View

Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Is there a certain way of making HEPES buffer w/ KCl?

I am trying to switch from NaCl to KCl in my buffer. As soon as I add 4M KCl solution to DI water and HEPES, it precipitates. Would heating the buffer alleviate the problem? Or should i add KCl to...

07 January 2021 1,104 1 View

What are some of the differences between a research article and a review article?

What are some of the differences between a research article and a review article? Thanks in advance for your replies!

29 December 2020 2,066 26 View

For experts in Gaussian 09 for windows?

Dear Friends: 64 bit Gaussian 09 software for windows is more expensive than 32-bit Gaussian 09. In our work, we deal with coordination compounds with about 50 atoms. So, can we use 32-bit...

27 December 2020 2,877 2 View

Suggestions for Journals related to Psychology and Computer Science?

I am looking for a journal, related to Psychology and Computer Science. IF < 2.0. If anyone has published some related work, or know any journal. that would be nice.

07 December 2020 1,277 3 View

FEM modeling of Joints/ Connection?

The attached Joint.png showed and example case of a joint (2 continuum members are connected). There are 3 characteristics of this, 1. There is a Gap: Cause small amount of rigid body rotation and...

07 December 2020 6,603 3 View

How to compute intermolecular interactions in mixture of solvent?

Hello Is it possible to compute intermolecular interactions in a mixture of solvent using DFT tools in Gaussian-09 or GAMESS US, DMOl3 or any other computational chemistry code? For example we...

05 December 2020 7,336 3 View

How can we set up Hydrothermal Liquefaction using solar/microgrid for extracting biofules?

Fo the conversion of biomass to biofuels, Hydrothermal Liquefaction is chosen.But the whole operation has to be from renewable energy source including the whole process most probably by solar and...

23 November 2020 9,432 2 View

Do you think that the emergence of CORONAVIRUS affects Investor trading behaviors? How? give examples?

any Thoughts on this question will help me Thanks

06 November 2020 7,654 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View