During Fermenation at 10L scale Pichia Muts, We observed rise in pH level from 5.0 to 6.0 and above , whenever there is increase in DO level. we kept set point for DO to be 70% and wait till DO spike to 90 and then we gave glycerol feed.
Can anybody explain is there any direct correlation between pH and DO levels in this case?
Abir Chakraborty
which vector are you using? Do not you have the provision of using AOX1 promoter as AOx1 is more tightly regulated.
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Pravin Sapate
We are using the Muts strain, wherein the AOX1 promoter is disrupted, and we rely only upon the AOX2 promoter for methanol metabolism.
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Diego Noseda
MUTs consume less methanol so the consumption of dissolved oxygen decreases during the fermentation time.
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