I am trying to integrate a 11 kb DNA, linearized with AvrII, at GAP locus in Pichia pastoris genome. I am using electroporation for the same. After transformation, I am observing colonies after 2-3 days but in test plate as well as negative control. I have tried using different concentrations of the antibiotic selection marker as well (100, 200, 400 ug/ml) but I am facing the same problem. Can anyone please suggest any solution for this?