What should be boundary condition on wave function in a continuous potential?

If the potential is a continuous function of x ,say V,(x)= - A ( exp(2|x|/a) -1) ,what should be boundary condition on wave function ? Further ,will it be correct to argue ,wave function and...

11 December 2020 6,617 4 View

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA?

How to calculate antibody titre in serum of vaccinated animal using Direct ELISA? The OD values obtained at different dilution how to determine the cut off OD against which the antibody titre...

31 January 2020 909 4 View

DNA precipitation without ethanol?

Is there a way of precipitating DNA from water without using ethanol? for example, mixing DNA with glycogen and ammonium acetate and then spinning it long enough so the bound glycogen+dna would...

22 August 2019 5,501 6 View

What is the meaning of writting a comment on a published paper ?

When one writes a comment on a published paper , what is actually the intention of the commenting author ? To point out the mistakes or present complete solution . It is seen that very often...

25 February 2019 6,029 4 View

Multiple peaks of the same compound in LC-MS Analysis?

Dear All, I was analysing some MAAs (mycosporine amino acid like substance) samples. HPLC shows a single peak with RT 4 wavelength 333nm. so I thought it is a pure compounds. To know the mass of...

21 August 2018 9,698 3 View

Silicon atom types in Charmm36 ff?

Hi, I want to simulate a ligand containing Silicon atoms ( present in the aromatic form as well). I wanted to ask if anybody could let me know the presence of silicon atom types in Charmm36...

14 June 2018 4,648 3 View

Can I use PVP instead of Sodium acrylate for the synthesis of graphene oxide magnetite nanocomposites?

It says that sodium acrylate is used as a stabilizer and to improve solubility of the precursors. And without its presence the particles aggregate. Can I use PVP instead??

12 June 2017 7,979 3 View

Can you help in MATLAB for H=p^2 - x^4 +2ix on eigenvalues ?

Can you help me in eigenvalues calculation using MATLAB for H=p^2 - x^4+2ix ?

29 June 2016 5,501 2 View

What is an index value in a gray scale image which is not in an rgb image?

When i convert an rgb image to a gray scale image in MATLAB, there is an index value in the gray scale image but for the rgb image.

03 April 2014 1,831 1 View

Why we are reluctant to admit own mistakes ?

It is seen that some people are reluctant to admit their own mistakes . Do they feel their social dignity/ prestige are in danger ? On the other hand they should know that one can make fool to...

01 January 1970 8,902 63 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View