17 December 2020 0 5K Report

I would like to detect late endosomes in cell and use AF 594 labbled LAMP-1 antibody (Invitrogen). I found out that the signal of LAMP-1 was brighter in the samples that were not permeabilized by Triton-X 100 (0.1%). Confocal micrpscope was used. I want to know whether Triton distribute the membrane system of cells and induced the unexpected negative results or not. I have searched some references read that permeablization was conduced.

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