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Questions related from Lan Mei
As mentioned in the title, how to maintain cells in hypoxia conditions? The special incubator is not always available. Furthermore, I would like to keep cobalt chloride away, a commonly used...
28 November 2021 5,734 3 View
Both the two cells are TNBC cell lines? What's the differrences between them? Especially, the differences of the sensitivity to photodynamic therapy. It is appretiated if references will be attached.
07 November 2021 8,584 3 View
I would like to detect mycoplasma contamination in cell cultures. Could someone provide me relevant protocols and references? Thanks.
20 October 2021 2,501 3 View
I am conducting experiments about enzyme kinetics. And I am confused about data normalization. For example, Uv absorbance of the enzymic reaction is usually used to monitor the process. I have...
08 July 2021 6,803 2 View
Both DPBF and ABDA could be used to probe singlet oxygen. But I am not sure wether the two prober could be used to detect intracellular singlet oxygen or not. Could someone share me some references?
24 June 2021 252 1 View
I would like to develop a co-culture modle using a airyway epithelial cell and a bronchial fibroblast. I have read some references suggesting that I should seed the epithelial cell in the upper...
02 June 2021 831 3 View
I am trying to use tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) chloride to detect intracellular molecular oxygen. However, a few references could be found out. Can someone give me a...
29 May 2021 3,334 6 View
I would like to use FRET effect to probe the distance between two functional moieties (I am sure the distance is smaller than 10 nm). I have tried several times, but I found out that the FRET...
22 February 2021 3,730 1 View
I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...
24 December 2020 8,560 4 View
I would like to detect late endosomes in cell and use AF 594 labbled LAMP-1 antibody (Invitrogen). I found out that the signal of LAMP-1 was brighter in the samples that were not permeabilized by...
17 December 2020 4,526 0 View
I would like to manipulate four different cells for confocal imaging in order to observing the endosytosis of a nano-scaled drug payload. The culture medium without phenol red is preferred. But...
28 November 2020 5,907 0 View
I am preparing a co-culture experiment in which transwell inserts will be used. According to the references, 0.4 micrometer PET membrane was selected. And different drugs were added to the upper...
16 November 2020 3,839 2 View
I would like to measure the permeability of my drug using in vitro model, transwell, specifically. However, I only have the cell line, not the primary cell. Can I use the cell line to plate in the...
09 November 2020 3,469 1 View
Triton X-100 has been widely used to permeablize cells for flow cytometry. How can I choose the concentration of Triton X-100 if I would like to detect expression of one protein in the whole cell?...
01 November 2020 643 1 View
I am working on the culture of tumor spheroids to evaluate the permeability of a polymeric drug delivery system. I would like to take CLSM images of spheroids when they are alive. Is it resonable...
25 October 2020 1,065 2 View
What's the different between paraformaldehyde and glutaraldehyde when they are used as fixers? I have prepared cell cover slides and fix them in 4% paraformaldehyde solution. Then 0.1% Triton-x...
08 June 2020 5,592 0 View
I need to translocate my cells from one labrotary to anther for live cell imaging, several tens minutes are needed for the translocation. The cells have to be removed from the incubator, and how...
19 May 2020 7,781 5 View
I am working on the MD simulation of 3D self-assembling nanostructures. But researchers in our group have mainly worked on proteins, small molecules and synthesized polymers. Softwares including...
01 April 2020 9,811 3 View
I would like to make MD simulation of a 42-bp B-DNA double helix, which has a segment of single strand at each end of the chain, and a small molecule anti-cancer drug. I have searched PDB and NDB...
10 March 2020 3,717 3 View
I have read ref about MD simulation of interactions between small molecules (drugs) and DNA. I am really a green hand in MD simulation, and don't know how to chose a software. Is AMBER my best choice?
03 March 2020 7,060 4 View
I stained MCF-7 breast cancer cells with DID following the manufactorer's protocol (20 nM for 20min). However, it is found out that some organelles were stained besides the cellular membrane. I'm...
03 October 2019 2,482 4 View
How to deal with mycoplasma contaminant in cell cultures? The growth of my cells was obviously hampered.
21 August 2019 8,857 2 View
I used Native PAGE to examine the DNA self-assembling nanostructures after annealing. I run my PAGE in a ice bath and 1X TAE was used as buffered system. The gel had been pre-electrophoresised...
20 August 2019 7,939 3 View
I am testing antibacterial properties of a peptide of which the mass is as low as tens of microgram. If I want to set a wide range of concentration, I have to dissolve the peptide powder in a...
18 May 2019 4,851 2 View
