How can I simulate noise of CMOS Image Sensor by sentaurus TCAD?

Can anyone tell me how to simulate noise of CMOS image sensors, such as thermal noise and flicker noise with sentaurus TCAD?

09 November 2020 9,616 4 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Can I put one mouse in one cage?

I have three mice but I only need to scarify two in my experiment. Can I keep the leftover one mouse in the cage with itself? The other option is to put it in another cage with other mice. But...

12 July 2020 307 5 View

Why ammonium chloride an inhibitor for lysosome?

Ammonium chloride is a lysosome inhibitor and a weak base as described in a published paper, Ammonium chloride, an inhibitor of phagosome-lysosome fusion... . However NH4+ is a weak acid, I...

21 April 2020 8,419 3 View

How to design steel rack accorfing to ANSI MH 16.1: 2012?

Does some have got the commentary of the American norm ANSI MH 16.1: 2012? according to this norm the seismic design of the steel rack could be performed using R=4 as behavior factor, this value...

19 November 2019 6,598 2 View

Why I have very strong phospho protein signal in western blot?

I am working with phospho protein in western blot and have problems about the phospho protein signal. It is common that researchers can not detect phospho-protein signal in the membrane, as...

17 July 2019 6,027 3 View

How to decontaminate a 2D materials on TEM grid for atomic scale imaging?

I was trying to imaging the 2D materials with aberration corrected TEM, including MoS2, ReS2 and graphene. However, I found serious carbon contamination when zoom in at the region of interest....

30 May 2019 9,828 3 View

How to use software Geneious to find genomic features?

How to use software Geneious to find genomic features such as genome size, GC content, tRNA genes, CDS etc?

10 June 2017 1,062 1 View

How canI verify the DNA fragment without results after blast in NCBI?

15 January 2017 4,595 3 View

Does anyone have any reference about only using fluent and ansys workbench static structural to do a two way fsi analysis for a propeller?

Can anyone give me a hand to share some reference about this problem and I will be very thankful for that.

05 February 2016 6,931 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View