Dose anyone know the exactely steps to produce humanized monoclonal antibody?

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How to create ITO materials in FDTD?

Here are the ITO material parameters and the Drude model I found in an article, but I don't know how to import these parameters correctly into Ansys lumerical FDTD.The attached picture shows the...

18 June 2024 3,182 10 View

How to create ITO materials in FDTD?

12 June 2024 5,915 2 View

How to set the background of plot to white color in Silvaco Tonyplot?

Figure 1 is my plot, the background will be colored when the quantity is set to electron concentration. While Figure 2 is the figure i want. How to set the background to white color when the...

11 June 2024 8,621 1 View

How to calculate the number of Zn atoms on the surface of ZIF-8 particles?

I would like to replace the Zn atom on the surface of ZIF-8 particle by the ion exchange. I have the XRD and TEM data of ZIF-8, how many Zn atoms on the surface of this particle?

18 September 2023 5,138 3 View

Regenerative energy system in EV will be a reserch hotspot in the future?

20 August 2023 8,125 1 View

Why is plasmid transfection not effective?

Hello everyone, I am currently doing transient transfection. I want to transfect the overexpressed plasmid into HCC cells, and this plasmid has 10000+ base pairs. The transfection reagent I used...

15 July 2023 3,881 3 View

Is there a solvent that only dissolves Polycarbonate but does not dissolve Poly(propylene carbonate) ?

When I do the PPC-PC reverse dry transfer method, PPC and the sample fall on the PC, and then I melt the PC/ sample /PC onto the silicon chip, how do I remove both PPC and PC to get the sample?

01 July 2023 4,026 1 View

How to simulate 1/f noise in MOSFET with silvaco?

In atlas simulator, i have add interface charge with "interface" keyword, Then i start a noise simulation on this MOSFET with interface charge, but the PSD of 1/f noise shows zero with the...

01 June 2023 4,710 0 View

How to solve equation "xy + ax + by = c" for integer solution of x and y ?

It is well known that the equation in the form of "ax + by = c" (where a, b and c are known integers while x and y are unknown integers) is called linear Diophantine Equation (LDE), and has been...

06 March 2023 1,514 23 View

What does it mean when the relative intensity of XRD peaks changed?

When we synthesized the same compounds at different temperatures, the XRD peaks appeared at the same positions. But the relative intensity of the characteristic peaks changed. For example, the...

06 October 2022 2,950 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View