Can nonanol be directly oxidized into nonanal by O2 under ambient conditoions without any catalysts or additives?

Can nonanol be directly oxidized into nonanal by O2 under ambient conditoions without any catalysts or additives? any comments and literature would be appreciated.

09 November 2020 6,744 5 View

How to use pilot data to do simulation to calculate the sample size for linear mixed model using matlab?

Hi, all, I have a 2(group)*3(time) design, and at this moment, I got 5 subjects in each group in the pilot study. I would like do a power analysis and sample size calculation. since the design I...

03 February 2020 8,851 1 View

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If the mass and volume of the oil droplet are known, and the oil droplet is formed from nonanoic acid, how to calculate the cross-sectional area and thickness of the oil droplet on the water...

28 December 2019 705 3 View

How to present the relationship between two mutually exclusive independent variables on dependent variable in one model?

I want to compare the different impact of two communication strategies (stra. A and stra. B) on brand trust. Meanwhile, the main effect between communication strategy and brand trust is mediated...

20 June 2019 5,122 3 View

Why the distance between bromophenol blue bands and xylene cyanol FF bands are close?

I am doing the gel electrophoresis to check my DNA samples, and I found that after adding loading buffer and start running, the distance between bromophenol blue bands and xylene cyanol FF bands...

04 June 2019 2,300 3 View

What are the bandgap scope of carbon quantum dots with blue,cyan and green fluorescence, respectively?

What are the bandgap scope of carbon quantum dots with blue,cyan and green fluorescence respectively? such as red fluoresence carbon quanutm dots correspond to ca.2.06eV. How about the others?

18 December 2017 9,681 2 View

Why i cannot get a good curve of acarbose in alpha-glucosidase assay?

I had tested Acarbose in Alpha-glucosidase assay, the result showed a very low absorbance, it almost same reading as negative control (Distilled water). Below is my method of the assay. Anyone...

08 December 2017 2,457 2 View

Can ATP be separated from AMP an ADP by silica gel TLC plate? If so, what is the best developing system?

How to purify ATP in a large quantity, like 1 gram? Is it possible to run a silica gel column instead of a ion-exchange column? Thanks.

09 November 2017 5,943 5 View

My research show fungi grew in PDA contained high conc. heavy metal but did not grew in low conc. heavy metal, why this happened?

my research show fungi grew in PDA contained high concentration heavy metal but did not grow in low concentraion heavy metal, why this happened? Because in theory, the fungi did not grow in high...

02 May 2017 994 3 View

For Chip-seq, how to design primers for qPCR validation of purified DNA after imunoprecipation?

26 March 2017 1,435 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View