Hello all,
I am planing to use AAV virus to transduce mouse primary cultured cortical neurons. Can anyone have the experiences or I possible share the protocols to me?
For the PDMS material, it's linear thermal expansion coefficient is about 3.2*10-4/℃. The polyimide has a smaller linear thermal expansion coefficient like 2*10-5/℃, but it cannot be stretch. It...
04 July 2024 4,525 1 View
Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...
17 June 2024 6,911 2 View
I found a bottle of lyophilized cycloheximide in the lab refrigerator (4℃). I think lyophilized form should be rather stable, but it's been open for 3.5 years. Cell signaling says the lyophilized...
11 June 2024 8,366 0 View
Does anyone know the exact BS concentration of the Zymo DNA methylation gold kit? or is there anyway to measure the BS concentration by titration? Thanks.
05 May 2024 8,848 1 View
What are the 3’ end modifications that prevent DNA from being extended by DNA polymerase? Which one has the best blocking effect? Leakage is minimal?
01 May 2024 5,188 5 View
I am facing a problem with the simulation of the precast concrete beam with prestressing. Typically, a precast beam would be fabricated in the factory with prestressing tendons (precambering) to...
06 February 2024 1,046 1 View
Can BHQ quench the fluorescence of FAM in the case shown below?
28 January 2024 6,182 1 View
Do ants in the genus Lasius migrate? If so, what are some of the characteristics of their migratory routes? Cheers
03 December 2023 6,320 3 View
Hi, everyone. May I ask if anyone knows the specific mechanism by which carbon dioxide (CO2) passes through the cell membrane of methanogenic archaea, is it similar to that of bacteria?
13 September 2023 9,548 0 View
In SmartPLS, I used a reflective-reflective model. In the first order model assessment, the reliability is fine. And the validity is fine. My validity assessment included cross loadings,...
06 August 2023 1,636 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
04 August 2024 4,090 0 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
18 July 2024 2,420 0 View
While living in Boston, which has a fine metro system, there were times that I would exit from underground at the wrong location, thereby expecting a particular visual scene only to be momentarily...
17 July 2024 3,237 10 View
Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...
14 July 2024 9,852 0 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View