Dose anybody has used human CD38 MACS beads to sort murine cells? The structure of human CD38 is very close to mice CD38 but can somebody confirm to me that the antibodies directed toward the human CD38 can work on mice?
Since the monoclonal antibody to human CD38 used in the human CD38 microbead kit is a mouse IgG2a, kappa, I suspect it is highly UNlikely that it would bind mouse CD38.
But the way the CD38 kit works is that you label your cells with monoclonal antibody to human CD38 that is conjugated to biotin, then use anti-biotin microbeads to do the magnetic selection, you should be able to use Miltenyi's anti-mouse CD38-biotin, which is a rat monoclonal antibody, then use the anti-biotin microbeads to run on your MACS column.
The product number is 130-101-947, 30 micrograms in 1 ml. I don't know what amount to use, although I suspect it would be 100 ul per 10^8 cells. You can ask Miltenyi's technical support people about that.
Alexandre Balaphas Perfect replies by both the experts Han Zhang & Gary Lee Gilmore
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