Effective and advanced materials?

1. What is the best concrete admixture that will enhance the strength of concrete? 2. What is best waterproofing materials that can be grouted in concrete structures? 3. What is best concrete roof...

11 September 2018 1,637 5 View

How do you choose a dye to label a primer for fragment analysis using a sequencer?

A primer is labelled with dye; for example FAM, VIC, NED etc for fragment analysis using a sequencer. We see that different primers are labelled with different dyes. Is there any basis to choose a...

14 May 2017 3,832 6 View

Can sequences of D-Loop mitochondrial DNA from various individuals in a species using different primers used for phylogentic studies?

There are different primers used in sequencing of D Loop region of mitochondrial DNA in horse. Will different primers not result in different sequences? and Can the sequences obtained using...

29 March 2017 6,063 6 View

Power of analysis_sample size in survey!

22 December 2016 7,700 3 View

What are some of the best ways to teach academic writing skills to students of second language learner (ESL)?

30 August 2016 8,878 29 View

Identify the species?

This species is a freshwater crab.

19 June 2016 1,714 7 View

How can we detect live or dead pathogens using PCR?

I am assessing the effect of Thermotherapy on Citrus Huanglongbing pathogen in root zone. PCR test result is still positive but I am not sure if there are any approach to identify if the pathogen...

31 May 2016 5,816 11 View

How can we make deconjested rootless NJ tree and make the taxon readable in MEGA 7?

I have constructed Neighbor- Joinning tree using mtDNA D-loop sequences from 92 horses using MEGA 7. The dendrogram obtained is so conjested and the sequences unreadable. How do I make the...

01 January 1970 878 2 View

Does this insect happen to infest on paddy?

A new species of genus Macdunnoughia Kostrowicki (Lepidopter...

01 January 1970 8,058 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

How to correctly choose the number of solution intervals when preforming harmonic analysis in ANSYS?

Good day, I design the rail-wheel geometry and the harmonic frequency ranges between 20-10kHz with 500Hz solution intervals and the results makes sense. Most of the time on the related literatures...

19 February 2021 3,190 3 View

Is an issue for serial correlation when it is not found with the xtreg, fe on its lag ,but the lag become significant when you've vce(robust) std. er?

Dear Sir/Madam I have a dateset that suffers from heteroskedasticity and serial correlation. To address the serial correlation lag my variables of the fixed effect regression. I retrieve the...

15 February 2021 3,700 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

How to best represent qPCR DD Ct or D Ct as a box plot?

I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...

11 February 2021 7,228 3 View

How do I keep duplicates based on specific variable in STATA?

Hello everybody. Im new to STATA and this forum but I hope you can help me. I am doing a research project where I intend to estimate the prevalence of venous thromboembolic events (vte) up to 30...

24 January 2021 665 5 View

Which is the correct method to show Standard Error in graphs for factorial experiment?

20 January 2021 917 2 View

Is it necessary to have internal standard in Bio analytical Method Validation in HPLC?

Please suggest a suitable reference from I can get information regarding the internal standards and other calculations for bioanalytical method validations for HPLC.

19 January 2021 5,680 5 View

At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View