We are going to perform three OF behavioral analysis in 1, 6 and 15 month-old Wistar rats. However, we are in doubt as using the same squared area for different sized animals. Is there any gold-standard for OF test troubleshooting, especially regarding area per animal? If there is, does changing the OF size for larger animals still a valid way to prevent area-dependent influences, or must I keep the very same paradigm for all animals?

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