JASPAR db align TF PFMs to a sequence and its reverse, but not the inverted seq.
Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...
25 February 2021 9,002 13 View
This is to support or serve as a guide for my thesis regarding 21st century teaching strategies. Thankyou so much in advance!
07 December 2020 8,440 4 View
I need to include the bending mode dynamics in my simulation. So far, I am only considering the rigid body dynamics. So I need to implement the following equation: q'' + Kg*q = Phi*F(t), where:...
19 November 2020 4,270 5 View
Hi fellow researchers, Currently i'm doing some research in fungi-secreted phytohormone-like compounds (possible bioactive ones), and i want to know which method (for example, HPLC or GC-MS)...
06 March 2020 9,946 3 View
What are the most recommended books/articles in these areas?
17 February 2020 4,250 5 View
Hi everyone, we are testing a calibration-free algorithm to estimate the concentration of a unknown material using the LIBS spectra. It is implemented in Matlab and we plan to publish it in...
01 October 2019 3,202 1 View
What are the possible etiologies, signs, symptoms, etc., of this syndrome? What is the impact on the subject awareness, state of mind, and brain function?
31 July 2019 1,801 2 View
I am designing a pbr reactor for the production of sinteis gas The data are: GHSV at stp: 5.1 * 10 ^ 5 cm3 g-1 h-1 conversion: 0.751 Flow of food: 15083 kg / h time on stream 4h Catalyst density:...
21 April 2019 8,924 1 View
Good evening, I wanted to ask you about something I have been observing. I work in a laboratory that does cell biology and molecular biology and we usually have some reagents and kits in the...
11 April 2019 1,229 3 View
Hello everyone! I'm looking for information about edge effect and I want to start reading the pillars about this topic, especially its influence on the vegetation structure and environmental...
01 November 2018 5,225 5 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...
25 February 2021 3,292 3 View
I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...
13 February 2021 7,217 4 View
Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...
11 February 2021 8,421 13 View
Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...
10 February 2021 5,863 2 View
I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...
08 February 2021 1,957 4 View
Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!
08 February 2021 6,909 3 View
I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...
05 February 2021 2,530 3 View
Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...
05 February 2021 292 3 View
I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...
05 February 2021 9,834 4 View