I'm doing pH imaging to investigate pH regultion in cardiomyocytes. To adress the question of the role of NHE, I want to block it selectively. Previously I used EIPA for that, but when I did experiments with the membrane bound pH sensor DHPE fluorescein, I came to know that EIPA is showing fluorescence when excited with 405 nm laser (see also: Gold et al., 2010: "A Clathrin Independent Macropinocytosis-Like Entry Mechanism Used by Bluetongue Virus-1 during Infection of BHK Cells").
So, now I'm thinking to use another inhibitor such as cariporide, eniporide, sabiporide or zoniporide (Avkiran, 2008: "Blowing off acid: a new tool to study Na+/Hco3- co-transport). Does anyone have experience with fluorescence of those inhibitors?
I would look up the structures of these compounds and see if you find any pi-conjugated aromatic rings. If you find some, you can bet you'll find some sort of fluorescence. If you see that, I'd call a sales rep from the companies you may buy them from and see if they have any information for those particular chemicals, or if you already have access to them, stick them in a fluorometer.
If you're stuck with those compounds and they all fluoresce, maybe you'll get lucky and one of those absorb away from your 405 laser. If not, you may need to redesign your experiment.
Good luck.
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