Adjusting results in SPSS

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Why does a particular point mutation serine to alanine lead to significant change in protein mobility on SDS-PAGE?

I made several point mutations on a protein, and found that only one particular Ser to Ala mutation leads to significant change in mobility of protein on SDS-PAGE (WB is attached). The drop in...

16 January 2020 3,661 4 View

Change in protein mobility on SDS-PAGE when Ser is mutated to Ala?

I made several point mutations on a protein, and found that only one particular Ser to Ala mutation leads to significant change in mobility of protein on SDS-PAGE (WB is attached). The drop in...

16 January 2020 8,844 4 View

Neural Networks in SPSS anyone?

Im analyzing the impact of different biomarkers and clinical risk scores on postoperative complications and Id like to test the importance that every factor has on the final outcome. Even if I add...

12 February 2018 2,701 6 View

Covariate adjustment in SPSS

How can I perform covariate adjustment in SPSS (or AROC)? Seems that there are no guidelines, the only thing I can find are theoretical explanations which are not quite clear. Thank you in advance.

23 May 2017 6,110 8 View

RNA and DNA isolation

I want to simultaneously isolate RNA and DNA from THE SAME Anopheles mosquito samples. I have been trying different variations of the TRIzol protocol (back extraction buffer, sodium citrate) and...

13 December 2012 751 11 View

Weird biosorption isotherm data

Hello everyone... I was trying to study the biosorption process of Pb onto bean shells, and I got this type of isotherm data... I have a problem fitting it, because no model seems to fit my data...

01 January 1970 5,639 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Ette Etuk

I hope that I understand what you mean. I think that it is possible to change some values of the data and get results for comparison. without adjusting the data. This is possible if the changes are not saved into the system to cancel the previous values.

Danica Markovic

Well, I did the ROC curves based on binary logistics (biomarker specificiti and sensitivity for postoperative mortality). But then one of the reviewers asked me to adjust the results for CIRS G score (comorbidities). I was wondering what would be the right way to adjust my results with the score he requested. Thank you.