fatigue failure S-N curve ?

Hello dear, I would like to do a fatigue experiment on the Ansys program for a particular human bone. I need S-N curve where can I find it away from practical experiences

31 July 2020 2,340 5 View

Medium change before transfection?

Hello, Is it necessary to change culture medium before transfection. My cells abruptly lose attachment upon medium change. Thanks.

01 June 2020 3,811 4 View

Does chaetomiun globosum have a direct effect on pathogenic fungi, or is it limited to stimulating plants to resist?

The role of chaetomium globosum in controlling plant diseases

22 May 2020 8,510 3 View

How i can calculate nano particles concentration from their OD , when their weight is unkwon?

silver nano

21 July 2016 8,846 3 View

HBx detection via Western Blot?

Hello everyone, I am trying to detect HBx protein from Hepatitis B Virus through Western Blot. Knowing that the molecluar size of this protein is 17kD can anyone give me an indication about the...

26 April 2016 6,580 2 View

Core and HBx proteins western blot detection?

17 April 2016 6,828 4 View

Is the permeabilization step essential in Rhodamine 123 mitochondria staining?

11 April 2016 7,012 4 View

Hbx snd core protein western blot detection?

Hello everyone, I am trying to detect hbx and core protein with western blot, is it better to use a 10% or 12% Sds-polyacrylamide gel? Thank you in advance

04 April 2016 2,948 3 View

Rhodamine 123 staining protocol for Flow cytometry?

01 April 2016 5,451 5 View

HCV and HBV real-time PCR quantification?

30 March 2016 6,983 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View