Peace, mercy, and blessing of good. I am trying to blast algal nucleotide sequence (purified using primer target 18S rRNA) and the result did not show any alignments as well the search for the conserved domain didn't show any result. Any suggestion?
Yes, the NCBI database includes algae. Make sure that you aren't accidentally choosing human or some other subset as a default.
What do your .ab1 files look like?
Good luck!
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