Clearly mention about which characters you are mentioning.

which characters?

Definitely all microbial qualities get destroyed.

If your query is wrt, soil microbial component,Disinfectants will definitely target soil fauna.

The disinfection in the autoclave alters the chemical and physical characteristics of the soil, in addition to eliminating the organic matter and microorganisms of the same

I want to keep the physicochemical characteristics of the soil and eliminate all microorganisms (bacteria, fungi, algae ...)

With the treatment in autoclave, all the microbiota is eliminated in such a way that the processes mediated by them in terms of transformation and recycling of nutrietes

  are going to stop, you can also destroy the structure of small organic molecules that may eventually be important in aspects such as cell communication between plant and microorganisms, hormones etc. can also alter molecules that play a role in the aggregation of soil, also the transformation of certain organic molecules could generate new undesirable compounds (eg microbicides or phytotoxic). thermolabile forms of some nutrients such as N and P can also be altered.

La mejor forma de desinfectar el suelo sin afectar características físicas y químicas es la radiación ionizante aplicando una dosis de 10Kgy

I want to study the effect of one or two bacteria on plant and soil behavior , without interaction with other microorganism

Dear Massakib,

Why not to work in hydroponic condition?

In this way, you can "build" yourself the substrate by adding the support (beads or sand). Then you can autoclave your system and keep it very well closed. Finally, you can add the needed nutrients for the plants by injection using 0.2 µm filters that eliminate all infections.

Further manipulation should be done under a hood and the seeds of the plants can also disinfected.

It is posivel with irradiation

Thank you all for your answers;

Mister Ramez

Maybe there is a part where i will work in hydroponic condition, but i want to study the effects of interaction between the three elements : soil-bacteria-plant, so i have to work with soil

This issue, we ahve already discussed at length at RG but under umbrella of solarisation/sterilization. Surely soil disinfection does change soil ecology a great deal , thereby , chemical and physical properties of the soil undergo changes , quite distinct from non-disinfected soils. Here you mean soil disinfection synonymous to sterilization..?

Mister Anoop Kumar

Yes I want to eliminate all kind of microorganism in soil but in the same time conserve the maximum of physical and chemical property of the soil.

Its very difficult maintain orignal soil chemical and biological properties , unless the autoclaving condifions are defined. Under normal autoclaving conditions, I doubt too many soil properties will change so drastically ....