You means that we must detect the MGMT expression of the other cancer cells before the medication.It is negative correlated to the sensibility of TMZ. But why does TMZ has a low toxicity in normal cells than in Giloma.
Dear Gao,
TMZ is a pro-drug and it does not work well in vitro.
You have to use it between 100 and 800 µM depending on the cell line you will used in vitro.
Bernd Kaina also demonstrated in an Oncogene article that you ahve to wait at least 5 days in vitro to begin to understand TMZ MOA.
As emphasized by Christopher, TMZ works well in vivo for oligodendroglioma (see Lamoral-Theys et al. here attached), astroglioma (see Mathieu et al., 2008 here attached) and melanoma (see Mathieu et al., 2007 here attached).
TMZ is "THE" standard treatment post-surgery and concomittant radiotherapy for glioblastoma patients (see the publication by Roger Stupp and colleagues in Lancet Oncology, 2009).
Best regards
Robert
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