Is this possible if we can somehow neutralize the charge present on DNA to opposite by introduction of high positive charge "may be protein".
One strand is treated with high positive charge, such that one DNA double helix will be neutral for negative but extra positive ions present will provide net positive charge.
On the other hand DNA without treatment with positive ions stay negative as a net charge
Both sample are stained with two different fluorophore. WHen these DNA samples are brought together can these DNA ligate lateral to each other due to charge and we may get both fluorophore intensity to confirm.
Some very positively charged proteins such as protamine will bind to double-stranded oligonucleotides. The complex has a net positive charge. I did this in Dr. Delong's undergraduate research group in 2010 (Missouri State University). While it was our group's findings that the DNA wraps around the protamine, there was no evidence to suggest the DNA became positively charged overall enough to interact with more DNA. If more DNA were added, it would bind to the protamine.
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