Does someone know the antibiotic resistance for GV143 plasmid (Genechem, China) in bacteria?

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BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Para Antropólogos/as: ¿Quisiera ser entrevistado?

Hola, Soy una estudiante de primer año de la carrera de Antropología en Chile, siendo parte del alumnado de la Universidad de Concepción. Se me ha asignado junto con mi grupo el trabajo de...

16 June 2024 2,715 4 View

Can holes for pressure probes in journal bearings modify the original pressure distribution?

It is very common to measure the pressure distribution inside the bearings with pressure probes connected to the oil film through holes in the bush. Once filled and pressurized, the probe lines...

12 June 2024 1,936 0 View

Why do you translate my name?

My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.

27 May 2024 8,228 2 View

Anyone who knows how to program in Fortran or similar language who can access the complementary Incomplete Gamma function?

Hello Does anyone know how to program in Fortran or any other language that uses advanced calculation libraries? I need help making an executable to obtain results from 2 equations that use the...

20 May 2024 6,610 5 View

How to measure de expansion of the cumulus?

Hello, I would like to inquire about how the expansion of the cumulus can be measured during or after IVM. Thank you.

16 May 2024 9,330 2 View

The best media for IVP in mice?

Hello community! What is for you the best combination of media for IVM, IVF and CIV in mice? In my experience we usually use media made in our laboratory but I have the opportunity to purchase...

02 May 2024 2,548 0 View

BLAST sequence in plus/minus and plus/plus in reverse sequence when CRISPR is performed. Are they mutants?

We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...

25 April 2024 6,159 0 View

Determination of Tocopherols and phytosterols by GC-MS without derivatization?

Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...

22 April 2024 8,178 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Enquire about the calculation of percentage of reads mapped to the reference sequence?

Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...

20 July 2024 9,705 0 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View