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21 October 2020 2,969 1 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

21 April 2019 2,699 1 View

How to measure bacterial membrane potential by fluorescence measurement or imaging?

I'm interested in using fluorescence dyes to measure or image bacterial (Gram negative) membrane potential. I have tried PI (propidium iodide) before, but wonder if there are better methods or...

10 November 2018 3,341 2 View

Optimisation of multiplex PCR on cDNA template?

I want to detect gene expression by multiplex PCR. The multiplex PCR reaction works fine on genomic DNA, but on a cDNA template the same oligo mix seems not to work as nicely, although specific...

06 November 2016 8,559 5 View

Any publication about the cost-effectiveness of multiplex real-time PCR in pathogen diagnostics?

Hello everyone! Does anyone working in the field of molecular diagnostics (particularly using qPCR and multiplex qPCR) know an updated review or comprehensive research paper with data on the...

02 March 2016 8,537 1 View

What heat killing conditions do you use for bacteria?

02 March 2015 7,991 9 View

Which LPS would you consider "most typical" and suitable for testing in human and murine cells?

Hello, I am interested in testing the various effects of LPS in in vitro models of human (immortalized cell line) liver and mouse liver cells. I want to use an LPS that is enterobacterial and...

02 March 2015 927 7 View

What do you mean by "interpretability" in models?

Modeling is a process of building a useful representation of a system or phenomenon. Models have a purpose, for example to precisely simulate or predict the behavior of a system. Among other...

01 July 2013 1,792 17 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View