Does someone know how to separate fungal protoplasts from intact cells by centrifugation?

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Why are fungi inoculated on agar with the three-point inoculation method?

I think it is especially for moulds of the Aspergillus and Penicillium genera. I don't rmeember the reason! Do someone know? Thank you #fungi #agar #inoculation #moulds #aspergillus #penicillium

17 July 2023 6,661 5 View

Why are bacterial cells stored at -80°C and not at -20°C?

I noticed laboratories store bacterial cells at -80°C. I wonder if there any studies or anecdotal evidences showing that -80°C storage for bacterial cells works better, in terms of whichever...

21 October 2020 3,206 1 View

Does anybody know why male and muscle patients more susceptible to Covid-19 virus?

See enclosed WMS Covid advice

11 April 2020 2,926 6 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

21 April 2019 3,001 1 View

How to measure bacterial membrane potential by fluorescence measurement or imaging?

I'm interested in using fluorescence dyes to measure or image bacterial (Gram negative) membrane potential. I have tried PI (propidium iodide) before, but wonder if there are better methods or...

10 November 2018 3,482 2 View

Did anyone experimented the loss of the image focus with a confocal microscope using a microfluidic PDMS chip?

In my experiments I used a PDMS biochip seeded with cells and used confocal microscope to observe cells structures like tubulin, Golgi and so on. During the confocal acquisition in time,...

17 January 2018 8,305 6 View

Hello! has anyone expertise in estimating the lipid and glycogen reserves in small and microscopic nematodes?

Hello! I am working with filarial worms (particularly the infective L3 stage of Brugia) and I would like to know if anyone can recommend any method (possibly inexpensive) (or provide useful...

28 March 2017 10,012 3 View

Optimisation of multiplex PCR on cDNA template?

I want to detect gene expression by multiplex PCR. The multiplex PCR reaction works fine on genomic DNA, but on a cDNA template the same oligo mix seems not to work as nicely, although specific...

06 November 2016 8,739 5 View

Quick morphological differentiation of adult females of Anopheles gambiae sl and Anopheles funestus mosquitoes?

Hello! Is there anyone that can give some advice on how to quickly differentiate (morphologically) the adult females of Anopheles gambiae sensu lato from those of Anopheles funestus in the field?...

17 June 2016 1,314 5 View

What's the color of Ellman's reagent solution?

I am trying to prepare Ellman's reagent solution to detect Glutathione. Before mixing with my sample. The solution already has slight yellow color. Is that correct or I already contaminated...

09 March 2016 3,447 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View