METHOD: What method to use for transient gene silencing in Cucumis sativus ?

I'm planning to silence candidate genes in the root system of C. sativus and viral vectors seem scarce in Cucurbitaceae and with low efficacy. Therefore, I'm looking for people who have experience...

06 December 2020 1,716 1 View

How to define receptor atoms in MOE?

How can a set/define atoms as being part of the receptor for docking in MOE? I have a covalently bonded ligand-receptor complex, and I would like to perform a docking experiment with the complex...

18 October 2020 9,688 2 View

Is someone used a Single or double point method for protein intrinsic viscosity determination?

Dear all, I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins. These methods are usually developed for polymers and does...

06 August 2020 6,227 5 View

Dynamic light scattering instruments for several conditions in parallel?

Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...

14 December 2019 6,735 3 View

Why DHB is a reducing agent while sDHB matrix is not a reducing agent?

Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...

19 October 2017 190 3 View

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO?

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO? Ideally a questionnaire with a parent and teacher report version

08 February 2017 6,163 1 View

Why do cells round up upon DCFDA (ROS-staining)?

25 November 2014 4,325 6 View

Do you know any good french corpora for named entity detection?

I'm looking for a french corpus tagged with named entities in order to train supervised machine learning. Can you help me to find one?

06 March 2014 7,268 3 View

How can I get the specific RNA expression of an alternative transcript of a gene of interest?

04 November 2013 9,908 6 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

02 June 2013 3,473 20 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View