Monitoring wildlife road crossing points typically involves a combination of methods to gather data on animal movements and behavior. Here's a general methodology:Identify Crossing Points: Determine potential crossing points based on habitat connectivity, topography, and historical wildlife movement data. This could involve visual surveys, camera traps, or reports from local residents.Camera Traps: Install motion-activated cameras at strategic locations along the road, especially near identified crossing points. Set up cameras to capture images or videos of wildlife crossing the road.Track Surveys: Conduct regular surveys to look for tracks, scat, or other signs of wildlife activity near the road. Transect surveys can be used to systematically search for signs of wildlife presence.Remote Sensing Technologies: Utilize remote sensing technologies such as GPS collars or radio telemetry to track individual animals and understand their movement patterns in relation to road crossings.Roadkill Surveys: Implement roadkill surveys to document any animals that have been struck by vehicles. This can provide information on species composition, frequency of roadkill, and potential hotspots for collisions.Behavioral Observations: Conduct observational studies to document wildlife behavior around road crossing points. This could involve direct observation or the use of remote cameras to capture behavioral data.Data Analysis: Analyze the collected data to identify trends in wildlife movement, species composition, seasonal variations, and potential impacts of road infrastructure on wildlife populations.Mitigation Measures: Based on the findings, implement mitigation measures such as wildlife underpasses, overpasses, fencing, and signage to reduce wildlife-vehicle collisions and improve habitat connectivity.Long-term Monitoring: Continuously monitor wildlife road crossing points to assess the effectiveness of mitigation measures and track changes in wildlife populations and behavior over time.

Look up papers by Rick Engemann and Lee Allen. They published several on using tracking plots to assess wildlife occurrence.