Experiment or Quasi Experiment Design?

What kind of study design are we talking about? Subjects are randomly assigned to one of three groups. In addition, a moderator and a mediator are collected as continuous variables. Is this an...

15 January 2021 1,809 4 View

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

26 November 2020 4,609 15 View

Nvivo - how to extract and analyze hashtag?

Hello, I am using Nivo 12.0 plus. I've a dataset of tweets, unfortunately not extracted by NCapture. How can I extract and analyse hashtag? Is it possibile using auto-code functions? Thank you

19 August 2020 9,532 3 View

What is a scoping review?

In what context should we choose to do a scoping review? How is it different from systematic review, literature review, mapping review and Cochrane review? What are its mandatory or optional...

05 July 2020 8,206 3 View

If I use REVEL score as a meta-predictor for clinical variant classification, what would be a reasonable cut-off to filter disease variants?

Ioannidis et al., 2019, reported the development of the REVEL score (rare exome variant ensemble learner) for redefining pathogenic variant classification, and Tian et al., 2019, reported that...

29 March 2020 2,450 2 View

Is there a method to statistically compare two or more regression coefficients in paired data?

Hello, I have conducted simple linear regression for four time points, always on the same sample. My time points are 10 years apart and now I wish to compare the slopes / betas / regression...

21 March 2020 10,020 9 View

Is the Case Study Method eligible to create theory?

I have been conducting research for PhD Theses using case study as a method, mainly refer to Robert K. Yin works. At the final step of my research, I define some concepts and use the concepts to...

12 February 2020 3,146 10 View

What is the blue-shift in UV-vis?

hello, Im having trouble in extracting information from my uv-vis data. The sample the I analyzed is protein corona. Before that, I already characterize 40 nm of gold nanoparticle and obtain its...

15 May 2018 8,815 7 View

Please does anyone knows how to use genome mining in search of genes encoding for particular enzymes?

For instance if I want to search for genes encoding particular enzymes which bioinformatic tool is better and how do I select the best genes to investigate?

14 August 2017 1,967 4 View

Please i need your help ?

06 May 2017 5,743 7 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View