Does someone have experience with the determination of gamma-lactones in wine?

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What are the secrets of preparing nickel nanoparticles?

Hi, Research gate! I need HELP! I'm trying to prepare nickel nanoparticles for my devices. I use the commonly used method from the article (DOI: 10.1002/adma.201405391). Briefly, 20 mmol...

31 March 2024 7,168 1 View

Who has researched the talent management of contests talents in mathematics, programming, etc.?

Has there been any research on managing talents who have excelled in competitions in mathematics, programming, or physics/chemistry/biology? How can we ensure that they make their most valuable...

28 February 2024 6,003 0 View

Matrigel dilution for PDX?

To what amount can you dilute matrigel? It to inject PDX subcutaneously into mouse. Undiluted it's almost impossible to work with, but overdilution leads to matrigel being incapable to solidify....

23 January 2024 6,510 2 View

Is it possible to complete a Brownian dynamics simulation of proteins via the NAMD program?

Hello. The software which is used to simulate Brownian dynamics was already discussed here...

15 January 2024 7,904 0 View

Can it be non-enzymatic digestion of BAEE in enzyme kinetics determination experiment?

Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...

24 December 2023 9,368 1 View

What realistically maximal temperature can agarose solution reach when the tube is heated in boiling water bath?

Hello, did somebody check what could be the temperature of agarose in a tube when melted in boiling water bath?

15 December 2023 7,037 0 View

Can you provide me with an efficient plasmid extraction protocol from yeast?

I've been trying to isolate a low copy (CEN/ARS) plasmid from yeast that has selective markers for both yeast and bacteria (Trp and Amp). However, when I try to recover the plasmid I can't get any...

20 October 2023 6,749 2 View

How to compare parametric with non-parametric data?

I want to examine whether there is a significant difference between two data sets (the same number of participants, continuous variable). One has parametric distribution and another has...

06 September 2023 7,342 0 View

Pmel adoptive transfer: homozygous vs heterozygous?

Do I understand correctly that heterozygous Pmel mice have twice as less cells that carry anti-gp100 TCR? This should be right because of the allelic exclusion. If so, can one combine both homo...

05 September 2023 260 1 View

It is allowed to transform conference abstract into journal article?

I plan to present my findings on the conference. After that, I want to write a full-text article and submit it to the journal. About 90% of results will be new, while remaining 10% will be from my...

03 September 2023 4,109 3 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

MDCI module in Orca software?

Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...

21 July 2024 1,632 0 View

What are the principles of physical chemistry?

what are the main points

10 July 2024 9,236 5 View

What is the recommended type of pseudopotentials for QE?

Hello dear colleagues and professors Please what is the recommended type of pseudopotentials for QE? ultrasoft or norm conserving?? And from where get all types of pseudos (us, nc, pbe,...

09 July 2024 9,541 5 View

Is there any guidelines about zooming out a chromatogram?

Hi, Just curious to know that in chromatography, a chromatogram should be zoomed to what scale? 10 times of output , 50X or ..? Is there any regulation or general chapter which describes this?...

03 July 2024 3,119 6 View