I'm planning to perform some rapid preliminary experiments on epithelial sheets, such as staining for markers of baso-apical polarity and wound healing assay. Ideally I'd like a cell line that can be easily grown and transfected, so that I can rapidly get some observation. Importantly, I would need a cell line that form a nice epithelial sheet, to study the mechanical cohesiveness of the cell population.
Thanks a lot,
Pablo
Mh, osteosarcoma epithelial cell line U2OS might be the perfect thing: easy to handle, no fancy additives, grow like hell and are really easy to transfect. Or choriocarcinoma cell line Jar: they form a beautiful layer and are easy to handle and transfect as well. I guess Jars are often used polarity studies.
People oftentimes use MDCK cells for polarity studies. Fairly easy to use.
HCT116 is a great one. They're easy to grow - use McCoy's 5A + 10% FBS. They have epithelial morphology and grow like weeds. Cell doubling time is approximately 18 hours, and they're highly adherent.
http://www.atcc.org/products/all/CCL-247.aspx
African green monkey kidney (Vero) cells. Easily establish confluent monolayers and don't require special culture media. Also, Vero cells are very adherent but not super sticky making a multitude of experimental assays easily performed like flow cytometry, immunoblotting, and ELISA (to name just a few). Easily found on ATCC website too.
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