What are the necessary step and topics on how to break down your full report into a paper for publishing?

Hello, I wish to know how to break down a full report into a lesser number of pages that will fit publishing rather than publishing the full report of about 200 pages. So, what topics or main...

19 November 2020 5,990 7 View

Is there a need for the reliability of intsurments to be presented when submitting a grant proposal?

Our research project has been selected for funding and we are expected to provide a detailed proposal of the work. I am particuklarly confused if we should state how the reliability would be...

18 October 2020 4,702 4 View

Is it a good practice to publish in a journal, an article that appears in a conference proceeding?

Which of these two statements represents an acceptable practice? A. Publishing a paper that appears in conference proceedings in a journal is okay B. It is okay to publish a paper presented at a...

31 July 2020 3,633 5 View

Possible ways to separate two bioactive compounds obtained from a silica gel column that two dimentional tlc plate chromatogram has shown as isomers?

Compounds were obtained from silica gel column in very small quantity which could not be separated by prep tlc or on another column because one is not sure of getting anything at the end

11 November 2019 6,396 4 View

In CFA, Can I correlate error terms of different latent factors as suggested by modification index in AMOS?

I am using AMOS and have performed a CFA but my model has not achieved fitness. The modification index suggest correlation of several error terms. However, one of the pair of error terms is from 2...

09 September 2019 7,179 13 View

What are the problems in the sustainability of graduate programmes in Universities these days?

I am trying to present a problem of graduate programmes sustainability. However, i am a bit confused on what constitute poor sustainability of graduate programmes such as Masters and Ph.D. programmes

04 June 2019 8,727 7 View

Should single author papers be scored the same as papers with multiple authors?

During assessment, authors are usually scored based on their position in the list of authors for papers published. This is used for decision making and performance appraisal in Nigeria. Given the...

02 April 2019 7,523 16 View

Is it possible to run a moderation analysis using PROCESS by A Hayes in SPSS with a multidimensional moderator?

I would like to know if it is possible to run a moderation analysis with a multidimensional moderator in SPSS using the PROCESS macro

26 November 2017 7,189 1 View

Does anybody have a good antibody to detect human cells in mouse xenograph model?

my cells are human breast cell line GFP/luc but my GFP signal is very low. I cannot find a good anti-GFP to detect it (I also tried TSA amplification, it is not working). Anti-Luciferase antibody...

09 December 2014 2,156 19 View

Is there any bromelain from certain types of pineapple which is unstable (labile) at low temperatures but more stable at high temperatures?

I'm doing characterization of optimum temperature for bromelain enzyme from Bogor pineapple by measure the activity of the enzyme. I used casein 1% as substrate dissolved in universal buffer pH 8....

03 July 2012 3,380 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View