Does somebody have experience with eBlot™ Protein Transfer System GeneScript?

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Could I detect alpha synuclein aggregation using Native gel ?

Hi there, I've been trying to detect oligomeric bands of alpha synuclein, but I'm not sure if it might work because I have been told that the size of aggregates (beta-amyloid or alpha synuclein in...

03 April 2016 520 3 View

Do you have experience in HEK293 transfection in 6 well plates?

Hi all, can I transfer 2ug of DNA into 6-well plate of HEK293, is it toxic since HEK293 is sensitive? I am using PEI reagent (the gene that I transfect isn't toxic to the cells).

10 November 2014 4,379 27 View

Xhoi digestion

what is the recommended time for Xhoi digestion ???

08 September 2014 6,592 2 View

Does someone have experience with 9E10(sc-40) Santacruz anti-myc?

Hi all I want to know in which dilution can i use 9E10 (sc-40) Santacruz anti-myc antibody? and in which solution 1% ? skim milk or something else ? Thanks

08 September 2014 851 2 View

Can anyone recommend a SH-Sy5y transfection reagent?

Hi I would like to transiently transfect SHSY5Y cells. I know they are not very easy transfect and I read one article where they used Lipofectamine 2000 but on invitrogen protocol pages I did not...

07 August 2014 6,914 8 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View