Sleman Bsharat

6 Questions 24 Answers 0 Followers

Questions related from Sleman Bsharat

Could I detect alpha synuclein aggregation using Native gel ?

Hi there, I've been trying to detect oligomeric bands of alpha synuclein, but I'm not sure if it might work because I have been told that the size of aggregates (beta-amyloid or alpha synuclein in...

04 April 2016 488 3 View

Do you have experience in HEK293 transfection in 6 well plates?

Hi all, can I transfer 2ug of DNA into 6-well plate of HEK293, is it toxic since HEK293 is sensitive? I am using PEI reagent (the gene that I transfect isn't toxic to the cells).

11 November 2014 4,329 27 View

Does somebody have experience with eBlot™ Protein Transfer System GeneScript?

Can I transfer a large protein using this system (protein about 110-120 kDa)?

11 November 2014 6,010 3 View

Xhoi digestion

what is the recommended time for Xhoi digestion ???

09 September 2014 6,566 2 View

Does someone have experience with 9E10(sc-40) Santacruz anti-myc?

Hi all I want to know in which dilution can i use 9E10 (sc-40) Santacruz anti-myc antibody? and in which solution 1% ? skim milk or something else ? Thanks

09 September 2014 819 2 View

Can anyone recommend a SH-Sy5y transfection reagent?

Hi I would like to transiently transfect SHSY5Y cells. I know they are not very easy transfect and I read one article where they used Lipofectamine 2000 but on invitrogen protocol pages I did not...

08 August 2014 6,884 8 View