How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Which neuronal cell line contain D1R?

I'm formulating a drug delivery system that could stimulate Dopamine receptor, For that i need to choose cell line that contain D1R and could be simulated using my delivery system... I have read...

20 November 2020 9,104 2 View

What is Dopamine?How it affects human behaviour &directly connected to neuro-psychological disorders?

We all known Dopamine is a happy/joyous hormone secrets from brain.How it changes the over all human behaviour & responsible for the well being of humanbeing. Regards, Md Osim

02 October 2020 582 4 View

Dopamine transporter antibody?

I am looking for an antibody against dopamine transporter suitable for WB. I have tried a couple of antibodies but I obtained poor results. Can anyone help me?

18 May 2020 1,536 2 View

Can pramipexole be used instead of apomorphine for the verification of 6-hydroxydopamine induced Parkinson's disease mice model?

since apomorphine is not availble, and pramipexole and apomorphine are both dopamine receptor agonists, so I'm wondering if I can use other dopamine receptor agonists to verify the rotation test...

08 January 2020 152 3 View

What is the method of assessment protein in serum?

What is the method of assessment protein binding with radiolabeled compound in mice serum?

31 December 2019 4,456 4 View

Can pramipexole be used instead of apomorphine for the verification of 6-hydroxydopamine induced Parkinson's disease mice model?

since apomorphine is not availble, and pramipexole and apomorphine are both dopamine receptor agonists, so I'm wondering if I can use other dopamine receptor agonists to verify the rotation test...

31 December 2019 5,371 2 View

New methods for measurement hepatic insulin sensitivity both in vivo and cultured cells?

hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...

21 December 2019 6,557 1 View

New methods for measurement hepatic insulin sensitivity both in vivo and cultured cells?

hello everybody, I would like to measure Hepatic insulin sensitivity both in vivo and cultured cells. Thus, I am looking for new methods other than traditional previous approaches such as (glucose...

11 December 2019 4,911 1 View

Can we use HPLC-UV for measuring dopamine and serotonin?

Hi all, is it possible to measure dopamine and serotonin in mice brain using HPLC-UV? If yes, can you please share a protocol? Thanks in advance.

10 November 2019 704 3 View