We have a case where the MIB1 scores are low, in the bone marrow biopsy sample, when they were expected to be high and the controls are staining fine.
It depends on your decalcification method. If EDTA is used, you might have better antigenic preservation as with say acid-decalcification.m Loss of antigenicity is a major problem associated with decalcification.
dear Jan-Gret Nel,
thanks for your comment. We process the marrow biopsy according to the 'hammersmith protocol'.The issue was other IHC markes were staining well ( eg 79a) I was wondering if there was something about the MIB1 that is prone to poor staining on bone marrowbiopsy
Heavy metal fixatives (B5) plus acid decalcification kills reactivity completely. Zinc-formalin and formic acid decal solution reduces reactivity. Some remains but I don't know how reliable the result is. EDTA decalcification sounds like a reasonable approach.
- Badges
- Science method