Has anyone tried this?
I want to lyse whole blood samples, but I'm not finding a protocol for it using RIPA buffer.
I found a recipe for a lysing buffer, but it uses different salts (which I don't have on hand naturally).
How do new institutions become listed on ResearchGate, please? Where can you add the logo and details for other researchers to find your university or college?
30 May 2024 5,836 2 View
I am working on a long-term research project on how tourism providers and DMOs can create the environment for slow tourism across the English Channel ( La Manche) for visitors travelling to the...
04 March 2024 9,394 0 View
I am looking for a table or list of levels of question type for posing when teaching using the dialogue method. I am using dialogue journaling, where informants, peers and the lecturer all have...
08 February 2024 1,305 0 View
Does anyone know how long the small animal imaging contrast agent Exitron nano 12000 is shelf stable? My lab is trying to determine how much to order at once but I can't find this information...
10 October 2023 7,118 1 View
Are you using, or have you seen any journal articles where tacit knowledge is elcited using dialogue and journaling with the stakeholders, please? We have begun to use and now develop, this...
19 January 2023 1,304 0 View
Do you know of any work or any publications on literary tourism in Cherbourg? Or any data on ferry passenger arrivals and overnight stays in Cherbourg-Octeville? Do you know of any writing on the...
21 February 2020 3,582 5 View
Currently observing abnormal morphology and difficulties with attachment
25 July 2018 5,528 1 View
Studying travel writing often means analysing the work of established authors but has anyone worked on developing their own travel writing or taught travel writing as part of a tourism or English...
18 February 2018 5,369 5 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View