Does remaining RNA effects the cell cycle histogram in PI staining flow cytometry?

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How do you dissolve acetone precipitated antimicrobial peptides?

Hello everyone, I am trying to isolate antimicrobial peptides from plants. After precipitating the peptides with acetone, most of the pellet (peptides) did not dissolve. I used dH2O/PBS to...

14 December 2023 9,124 2 View

After staining DNA with Etbr, can silver staining be performed on the same sample?

I am studying DNA-ligand interactions with EMSA. Sometimes I cannot see the DNA bands in the PAGE gel when I stain it with Etbr. As silver staining is more sensitive to EtBr, I have to use the...

31 October 2023 6,075 2 View

What are the causes of not increasing amper in Non-denaturing PAGE?

Hello everyone, I wanted to visualize my DNA fragments after PCR in Non-denaturing PAGE, however I could not. Because amper was not increased during the PAGE assay. what are the causes of not...

02 November 2022 7,159 4 View

Is there any alternative method to isolate antimicrobial peptides from plants without using protease inhibitor cocktail ?

Hello everyone, I want to isolate antimicrobial peptides from plants but, I don't have a protease inhibitor cocktail right now and possibly not in the near future. Is there any alternative...

28 August 2022 5,037 3 View

What is the difference between MUSCLE and CLUSTALW in aligning nucleotide sequences?

Is it better to use MUSCLE or CLUSTALW to align nucleotide sequences of genes belonging to the same genus?

02 June 2022 2,028 3 View

How to stabilize a fumed silica dispersion?

Hi, Does anyone have any experience / advice on stabilizing solutions with heavy fumed silica loading (~60%)? I am seeing odd viscosity behavior in my system where stored solutions are showing...

01 June 2022 4,527 5 View

UV Cationic Epoxy Cure - Is Shrinkage Dependent Upon Cure Conditions?

Hi, I am looking to see if I fully understand cationic epoxy cure and shrinkage. I have a highly filled cationic epoxy adhesive designed to achieve minimal shrinkage upon cure with UV light. My...

09 November 2021 7,435 1 View

Young's Modulus and Storage Modulus - In Simple Terms?

Hi Everyone, I know this question has been asked a couple of times here: what is the difference between Young's modulus and storage modulus? But the reason hasn't sunk in for me yet, so I am...

26 October 2021 9,844 6 View

How stable are polyurethane coatings in strongly acidic conditions?

Hi! I am trying to find some studies (or be educated) on the stability of the following groups in highly acidic (pH ~ 1-3) aqueous solutions of either an oxidizing acid (sulfuric or nitric) or...

29 March 2021 4,643 4 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 8,109 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View