We want to try the ribopuromycylation method for locating active translation in cells e.g. David et al., 2011. JBC. 286. 20688-20700
Yes, 1- 10 µg/ml works good...
We are also interested in using puromycin for quantitating protein translation in yeast. Is S.cerevisiae sensitive to puromycin or is a PDR5 knock-out strain required?
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
10 February 2021 5,927 1 View
If Lmn(x) is the Laguerre polynomial of order m and degree n Is there a means of computing Lmn(x) for 1) m goes to infinity 2) n goes to infinity
08 February 2021 6,320 4 View
Say for arguments sake I have two or more images with different degrees of blurring. Is there an algorithm that can (faithfully) reconstruct the underlying image based in the blurred...
17 January 2021 7,324 3 View
Hello everyone, Im doing a pushover Analysis of a cantilever beam in ABAQUS, made of reinforced concrete, but my model doesnt seem to converge proberly. The program simply aborts before hitting a...
15 January 2021 1,627 3 View
First time to use FE Panel Data Regression using LSDV, and not familiar with how to illustrate it in a research framework. We have regional CSR score as IV and Social Development ( four areas:...
14 January 2021 580 3 View
Who are the gods that ancient Egyptians thought of as closer to them and could have a personal connection with them?
25 December 2020 5,505 1 View
Greetings to all! I am humbly asking for some help, in regards to the checking of my moth samples. Though it is of great inconvenience to you all, I will respect your decision nevertheless....
08 December 2020 6,009 5 View
I am doing simulation exercise of which I have to inject fluid pressure into a borehole. The parameters I am using the injection rate is given in pressure (MPa) and the software accept injection...
01 December 2020 3,221 2 View
The textbooks show a triangular pattern for the current as the limiting case for a short dipole, and how this may be considered to be the limiting case for the sinusoidal current distribution....
26 November 2020 5,280 5 View
Next semester I am teaching an online class on Data Structures and Algorithms. The students will have had two programming classes in C++ and Java each. I am looking for a way to engage the...
11 November 2020 1,176 4 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View