Does puromycin enter yeast (cerevisiae) and what concentrations has anyone used?

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Which cointegration approach should I use when the variables are integrated in different orders?

I am working on a 21 sample size data time series, and after applying ADF test one of my variables are integrated of order 2 I(2), two variables are integrated of order 1 I(1) and two are...

08 June 2024 5,787 12 View

Can you give me an introduction about my research?

Cultivating a Love for Reading Amongst 21st Century Learners through Think, Pair and Share.

23 April 2024 4,916 0 View

If the curvature of spacetime at the beginning of the universe is infinite, how can the universe evolve to now in a finite amount of time?

According to the Big Bang theory, our universe began with a singularity, where spatiotemporal curvature is infinite. That means it would take infinite time to come to the present. That's...

19 March 2024 9,482 2 View

What should be the color of my solution after rotary evaporator?

Hello, I am currently doing a research about essential oil extracted using soxhlet extraction from guava (Psidium guajava L.) and my solvent is n-hexane. I am just wondering what should be the...

12 March 2024 4,525 4 View

What Should I Put on an XRF Table when Presenting a Paper?

I conducted an XRF test for my research and have a raw data file. However, I noticed that some of the chemical compounds such as K2O, Na2O, Fe2O3, etc. are missing, while their elemental...

02 March 2024 2,756 3 View

High voltage line noise and livestock?

I'm looking for research on the impacts of high voltage line noise on livestock. I tried the webpage search bar, but I keep getting pushed to a "are you a human" test and failing, sometimes even...

27 February 2024 4,395 1 View

What is the correct way to manage participants in a split head study?

Hello, My Masters research is a systematic review and meta analysis. One thing I am struggling with is when a study has undertaken a split head comparison (intervention on one side of the...

20 February 2024 257 1 View

Why does most laboratories still use gel separatory serum tubes over lithium heparin gel tubes?

The journal paper "The transition from gel separatory serum tubes to lithium heparin tubes in the clinical laboratory" discusses the advantages of lithium heparin over the traditional gel...

06 February 2024 7,581 1 View

Is it possible to remove multiple papers easily?

ResearchGate "found" lots of papers on which I'm not the author but share the same name with the actual author. There are over 100 of these! Is there a simple way to remove multiple papers or do I...

11 January 2024 6,531 2 View

What are good GPS trackers for recording the daily grazing movements of livestock?

I am looking for recommendations for GPS trackers to record the daily grazing movements of goats, cattle, and camels in the Mountains of Dhofar, Oman. In the past, I have used a tracker from...

18 December 2023 4,577 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View