I'm trying to learn about phycobilin-related fluorescence. I've learned that there is a pathway of phycobilin biosynthesis, which consists of reduction of hemin through biliverdin to phycobilin using enzymes oxygenase-1 and phycocyanobilin:ferredoxin oxidoreductase. I know that phycocyanobilin in complex with protein does fluoresce. My question is: does biliverdin alone, not complexed with any enzyme, exhibit any fluorescence? Also, would be great to get excitation/emission spectra.
Thanks!
In principle, Yes! It can be a fluorescent molecule. However, it would be necessary first to have a control viliberdin from a commercial source and record the excitation and emission spectra. In my opinion, the emission as well as excitation spectra is likely to change when it is associated with any other molecules.
I'm going to evaluate metabolites of HAPs in bile of fish and I have to measure content of biliverdin, so I'm going to realize in the next months assays to evaluate fluorescence of biliverdin(EX/Em wavelengths) to normalize content of metabolites. I hope that you have any information useful for me. Thanks!!
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