Sergii Pochekailov

7 Questions 3 Answers 0 Followers

Questions related from Sergii Pochekailov

Where can I physically get a large library of protein genes?

I am studying massively parallel protein-protein interaction. Is there a good source for a ready DNA library, where a protein gene (or part of it) would be surrounded by standard adapters? Like...

07 July 2018 8,561 3 View

Imagine you have a possibility to label each protein molecule with info which cell it comes from. What question can you answer using such method?

Imagine you can make cells express a protein and attach a "barcode" to each protein molecule. How you would apply such method? What questions can you answer?

04 April 2016 5,402 4 View

Is there a process of polynucleotide synthesis from nucleoside monophosphate, using energy from external chemical reaction?

Normally, DNA and RNA polymerization is realized by polymerases, using nucleoside triphosphates. The energy for the reaction is obtained from chopping off diphosphate group. I'm curious: is there...

12 December 2014 3,425 1 View

What are the exotic cases of bio-luminescence or bio-fluorescence?

I'd like to find as much as possible, information about biological fluorescence, luminescence or other easily detected property (color, etc.). Please don't answer with example of luciferase...

11 November 2014 8,060 5 View

Does anyone know examples of cytochrome P450 enzymes, which produce fluorescent or colored small molecule?

What I'm looking for is the cytochrome P450 enzyme, which accepts electron from adrenodoxin (or analogue), and produces fluorescent or colorful compound. For example, I already know about...

11 November 2014 3,054 5 View

Does pure biliverdin exhibit any fluorescence?

I'm trying to learn about phycobilin-related fluorescence. I've learned that there is a pathway of phycobilin biosynthesis, which consists of reduction of hemin through biliverdin to phycobilin...

11 November 2014 2,503 2 View

Can I make conclusions about certain protein redox states based on the redox state of cytoplasm?

Say, I have a protein in cytoplasm, which redox potential measured in vitro is +200 mV. The redox potential of cytoplasm appears to be say +50 eV. Knowing that, can I conclude that my protein is...

04 April 2014 1,747 1 View