1. Soil fertility status: if your soil is rich in nitrogen then nodulation will be poor or no nodulation
2. Soil rhizobium status: if rhizobium status is high in experimental site soil then ok. if not once again you have to inoculate externally otherwise no nodulation if occurs very poor response
3. Aeration and moisture: aeration and moisture are related to each other. Here excess moisture is present in soil without aeration then also root fail to produce nodule.
4. Soil temperature: definitely most important because if soil temperature is more then also poor nodulation.