PBS formulated with these ingredient separately , what will be its effect on cell Morphology or Viability? I am using PBS with Only Na2HPO4 as a part of formulation and using the same buffer for cell harvesting (Trypsinization) , will it affect the Viability of cells or wil it have any effect during Cryopreservation?
Adam B Shapiro
They are the same thing, once dissolved in water. Na2HPO4.2H2O has 2 water molecules per Na2HPO4 in the crystal form, which becomes part of the bulk water when the crystals are dissolved.
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Hathama Razooki Hasan
You can use either for the preparation since the final pH is 7.4.
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