Which sequence could induce higher immunogenicity, S polypeptide or only S1? and how to isolate S1 from a vector containing the whole seq. of S?

I have a clone of DNA sequence encoding the full spike protein (S1+S2)

01 January 2021 7,821 1 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is a good pretext task for self supervised lane detection in autonomous driving?

I have been looking into self-supervised methods in computer vision. For example Digging Into Self-Supervised Monocular Depth Estimation which looks at three consecutive frames of video, tasks a...

23 July 2020 8,232 1 View

What are some ways (aside from: a. mouse weight b. girth) to measure tumor growth in mice?

We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...

25 March 2020 5,991 2 View

Can anyone help me to find the psychometric characteristics for the JCQ VERSION 2 developed by Karasek??

I am using Job demands & control model in my thesis, and I want to use JCQ 2 questionnaire, but i cant find the psychometric characteristics

08 February 2020 2,240 3 View

Can we use a systemic NOAEL of a chemical product in a oral-administration effects study ?

Good evening, I have a already esablished Overall Systemic NOAEL (mg/Kg bw/day) for a pesticide wich i'll be studing the effect by oral administration on rats, can i use directly this dose in my...

08 November 2019 8,265 1 View

References about GA convergence or non-convergence?

I am looking for references to show that GA solutions do not necessarily converge to optimal solution to defend the use of an integer program, exact solution. I want to criticize heuristic and...

05 August 2019 900 5 View

In Data Envelopment Analysis (DEA), what to do if you are comparing with respect to outputs only?

Can we do that? And if yes, assuming that we have negative and positive output features, how to deal with them? By negative and positive features I do not mean value, but a feature like happy and...

22 January 2019 2,068 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

What questions should i include in my questionnaire?

I am writing my dissertation, about adults who trained at elite status in their youth, i am constructing a questionnaire with the goal of seeing, how many sustained injury, how many sustained...

28 January 2021 4,787 3 View

How to dissolve Scotch tape to collect the Au particles [analogous to the mechanical exfoliation of graphene]?

Hi everyone, I'm a mechanical person and now may need some help from chemistry experts. My question should be as easy as pie for the chemistry person. I prepared some Au particles in nanoscale...

21 January 2021 2,426 3 View

How commonly do you encounter Rummination Syndrome in your GI practice?

Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?

17 January 2021 5,998 0 View

Is it correct to include variables as a group and as individual component in multivariable cox or logistic regression?

For example : If I am conducting the effect of metabolic syndrome on short term mortality of disease xy, can I enter "metabolic syndrome" as one variable and its components (diabetes,...

11 January 2021 6,348 2 View

It does not appear logistically possible to vaccinate everyone for COVID. So what's the point?

Public health authorities claim that no one is safe from COVID-19 until every one is safe. This has translated into a goal to vaccinate everyone who can be vaccinated. Logistically, however, it...

06 January 2021 4,269 12 View

What's the reasonable loading control of nuclear protein in acute injury?

What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...

06 January 2021 1,607 2 View

Any one can tell me primer sequences used for q PCR to quantify MERS-CoV related coronavirus?

MERS-CoV primers

31 December 2020 2,389 2 View