I am looking to see if changes in concentration of NAC affect the afucosylation patterns in mammalian cells
Hi Muralidhar, if glutathione does not have any effect on glycosylation patterns then I doubt that N-AcCys will have any effect.
Dear In the UNIVERSE from a sand grain to Sun including man, all are bodies of different sizes. Every body has a SHAPE//FORM A body with a shape gets a NAME Every body has a LIMIT And every body...
18 May 2024 3,012 0 View
Dear, we draw a circle with the help of a compass , its tip of one of the leg of it as "Centre". Actually the beginning of the so called Centre becomes RADIUS. So, RADIUS EXISTS . And,...
02 March 2024 1,482 0 View
Dear,
23 January 2024 5,185 4 View
Dear in the past the entropy is low and with the arrow of time the entropy is high. Time moves from past to future and time never come back. Because , matter is made of many atoms. A perfume...
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Dear, I have a formula . Mail to: [email protected]
07 December 2023 7,760 0 View
I want to see the placental vascularization and angiogenesis in pregnant rats. thanks in advance
29 November 2023 7,634 0 View
Dear Every food item available in Nature have 1. Carbohydrate, 2. Protein, 3.Lipid, 4. Vitamins, and 5. Minerals. Their quantum varies. If one is a non-athlete the following may be followed (...
31 August 2023 8,294 6 View
Dear If you are 25 and above of age go for moderate eating. If you are 60 and above of age go for undereating.
13 August 2023 9,699 1 View
Dear, there are hundred and one food items in the world. Every item is rich in 1 Carbohydrates, 2. Proteins, 3 Lipids (Fats and Oils), 4. Vitamins and 5. Minerals. So eat ALL but restrict to 4...
13 August 2023 1,402 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View