In M. tuberculosis, cholesterol catabolism goes through a complex process. The ring opening process involves production of 3-hydroxy-9,10-seconandrost-1,3,5 (10)-triene-9,17-dione (3-HSA). A flavin-dependent monooxygenase hydroxylates 3-HSA to 3,4-DHSA. I wanted to find out whether M. smegmatis contains a homologue that carries out the hydroxylation reaction. If yes, what is the name of the enzyme and the gene encoding it?

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