Does lack of sucrose in MS media produce contamination or chemical precipitate?

Sam Lewis Champine-Tocher @Sam-Champine-Tocher

10 January 2019 1 6K Report

We are conducting an experiment with gametophytes of the fern Microlepia strigrosa at varied rates of sucrose in liquid MS medium. All of our 0g/L replicants are showing signs of what looks like contamination but could also be a chemical precipitant. The contamination/precipitant is not occurring in rates of sucrose 15g/L and above.

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