could any one help me regarding this question?
I'm currently working on the purification of protein process. There is endotoxin in final step of purification process. I applied different methods, anion exchange with q sepharose, ferric IMAC, liquid liquid extraction by triton x-114, ultrafiltration, applied DEAE resin, applied polymixinB, etc.... all of them weren't work properly to eliminate endotoxin.
I just want to test phenol against endotoxin in dialysis buffer or in industrial scale in TFF and buffer exchange. In some formulation buffer especially for disposable pen, phenol was considered as an excipient to prevent contaminant or some thing like that. Does phenol effect to degradation endotoxin in 5 or 10 mM concentration, when we use in dialysis buffer?
and second question, if we had endotoxin free protein and add 5mM phenol to the buffer of protein, does it effect to prevent creation contaminant especially endotoxin contaminant during storage time?
Thanks in advance
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