Elimination of yellow color from final product of protein?

07 August 2017 5,873 0 View

Increasing acicd isoform in protein solution

07 August 2017 2,084 1 View

Yellowish color of final h GH protein which was purified?

I am currently working on the His SUMO hGH purification process. After the purification of fusion protein, target protein undergoes a digestion stage and is separated from hGH using a SUMO...

04 May 2017 5,831 5 View

Does it correct apprroach to eliminate or degradate endotoxin from protein solution?

could any one help me regarding this question? I'm currently working on the purification of protein process. There is endotoxin in final step of purification process. I applied different methods,...

03 April 2017 3,893 0 View

Creation endotoxin in final sample!!!?

03 April 2017 702 9 View

Capture human EPO to the special IMAC resin?

I'm currently working on the purification of EPO which is secreted from CHO cell . for final step of purification I have to applied IMAC-Fe(III) for an especial goal.(Endotoxin removal) could any...

03 April 2017 8,515 10 View

Cleavage of fusion protein?

01 February 2017 5,153 4 View

I want to filtered cell's which is disrupted by high pressure homogenizer can anyone suggest me filtration system or facility instead of centrifuge?

After cell disruption in industrial scale, I want to eliminate cell's which is distributed, I should find some facilities such as filtration system, instead of centrifuge, could anyone please...

08 September 2016 9,949 4 View

Hello, could any one help me about a purification of protein process?

07 August 2016 9,557 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View